Preventive effects of intense continuous endurance training on isoproterenol-induced cardiac apoptosis and protein synthesis gene expression in wistar rats

Document Type : original articles

Authors

1 Department of Physical Education, Sharif University of Technology, Tehran, Iran

2 Department of Physical Education and Sports Sciences, Payam-e Noor University, Alborz, Iran

Abstract

Objectives: Heart failure contributes to cellular lesions and left ventricular dysfunction. The present study aimed to determine the effects of intense continuous endurance training on protein synthesis gene expression and prevention of isoproterenol-induced cardiac apoptosis. 
Methods: In this experimental study, 16 male Wistar rats were assigned to exercise and control groups. After eight-week treadmill running, with 15° inclination, at 65-75% of VO2max for 30-60 minutes, Isoproterenol (3 mg/kg) was injected into the rats subcutaneously for 7 days. And 24 hours after the last injection, the left ventricular tissue was stored at -80°C for qRT-PCR and TUNEL assay. Between-group differences were determined by parametric and non-parametric tests, using SPSS software version 24. 
Results: There was a significant increase in mTORC1 gene expression in the training group (P = 0.026) but AMPK alterations failed to be significant. eEF2K gene expression was suppressed in the training group (P = 0.001) which resulted in a significant increase in eEF2 expression (P = 0.005). Left ventricular weight and heart weight/body weight increased compared to the control group (P = 0.028, P = 0.010, respectively). And the training protocol effectively prevented the formation of isoproterenol-induced apoptotic cells (P <0.001). 
Conclusions: The exercise training protocol increased protein synthesis gene expression, and improved cardiac protection by reducing apoptosis. This protocol can be considered a promising modality in preventing and reducing apoptosis induced by heart disease such as myocardial infarction.

Keywords

References

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Cardiovascular Biomedicine
Volume 2, Issue 1
September 2022
Pages 42-50

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History

  • Receive Date: 24 October 2021
  • Revise Date: 16 January 2022
  • Accept Date: 04 February 2022

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